Randles, M.J., Humphries, M.J. and Lennon, R. (2017) Proteomic definitions of basement membrane composition in health and disease. Matrix Biol. 57-58, 12-28. PubMed

Horton, E.R., Humphries, J.D., James, J., Jones, M.C., Askari, J.A. and Humphries, M.J.  (2016) The integrin adhesome network at a glance. J Cell Sci. 129, 4159-4163. PubMed

Mana, G., Clapero, F., Panieri, E., Panero, V., Böttcher, R.T., Tseng, H.-Y., Saltarin, F., Astanina, E., Wolanska, K.I., Morgan, M.R., Humphries, M.J., Santoro, M.M., Serini, G. and Valdembri, D. (2016) PPFIA1 drives active a5b1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis. Nature Comm. 7, 13546. PubMed

Ajeian, J.N., Horton, E.R., Astudillo, P., Byron, A., Askari, J.A., Millon-Frémillon, A., Knight, D., Kimber, S.J., Humphries, M.J. and Humphries, J.D. (2016) Proteomic analysis of integrin-associated complexes from mesenchymal stem cells. Proteomics Clin Appl. 10, 51-7. PubMed

Horton, E.R., Astudillo, P., Humphries, M.J. and Humphries, J.D. (2016) Mechanosensitivity of integrin adhesion complexes: Role of the consensus adhesome. Exp Cell Res. 343, 7-13.  ScienceDirect

Horton, E.R., Humphries, J.D., Stutchbury, B., Jacquemet, G., Ballestrem, C., Barry, S.T. and Humphries, M.J. (2016) Modulation of FAK and Src adhesion signalling occurs independently of adhesion complex composition. J Cell Biol. 212, 349-64. PubMed

Mould, A.P., Askari, J.A., Byron, A., Takada, Y., Jowitt, T.A. and Humphries, M.J. (2016) Ligand-induced epitope masking: Dissociation of integrin α5β1-fibronectin complexes only by monoclonal antibodies with an allosteric mode of action. J Biol Chem. 291, 20993-21007. PubMed

Su, Y., Xia, W., Li, J., Walz, T., Humphries, M.J., Vestweber, D., Cabanas, C., Lu, C. and Springer, T.A. (2016) Relating conformation to function in integrin alpha5beta1. Proc Natl Acad Sci USA. 113, E3872-81. PubMed

Byron, A., Askari, J.A., Humphries, J.D., Jacquemet, G., Koper, E.J., Warwood, S., Choi, C.K., Stroud, M.J., Chen, C.S., Knight, D. and Humphries, M.J. (2015). A proteomic approach reveals integrin activation state-dependent control of microtubule cortical targeting. Nature Comm. 6, 6135. PubMed

Horton, E.R., Byron, A., Askari, J.A., Ng, D.H.J., Millon-Frémillon, A., Robertson, J., Koper, E.J., Paul,  N.R., Warwood, S., Knight, D., Humphries, J.D. and Humphries, M.J. (2015). Definition of a consensus integrin adhesome and analysis of its dynamics during adhesion complex assembly and disassembly. Nat Cell Biol. 17, 1577-87. PubMed 

Humphries, J.D., Paul, N.R., Humphries, M.J. and Morgan, M.R. (2015). Emerging properties of adhesion complexes: What are they and what do they do? Trends Cell Biol. 25, 388-97. PubMed

Jones, M.C., Humphries, J.D., Byron, A., Millon-Frémillon, A., Robertson, J., Paul, N.R., Ng, D.H.J., Askari, J.A. and Humphries, M.J. (2015). Isolation of integrin-based adhesion complexes. Curr Protoc Cell Biol. 66, 9.8.1-9.8.15. PubMed

Randles, M.J., Woolf, A.S., Huang, J.L., Byron, A., Jonathan, D., Humphries, J.D., Price, K.L., Kolatsi-Joannou, M., Collinson, S., Denny, T., Knight, D., Mironov, A., Starborg, T., Korstanje, R., Humphries, M.J., Long, D.A. and Lennon, R. (2015). Genetic background is a key determinant of glomerular extracellular matrix composition and organization. J Am Soc Nephr. 26, 3021-34. PubMed

Robertson, R., Jacquemet, G., Humphries, J.D., Byron, A., Jones, M., Warwood, S., Selley, J., Knight, D. and Humphries, M.J. (2015). Defining the phospho-adhesome: phosphoproteomic analysis of integrin signalling. Nature Commun. 6, 6265. PubMed

Byron, A., Randles, M.J., Humphries, J.D., Mironov, A., Hamidi, H., Harris, S., Mathieson, P.W., Saleem, M.A., Satchell, S.S., Zent, R., Humphries, M.J. and Lennon, R.(2014). Glomerular cell crosstalk influences composition and assembly of extracellular matrix. J Am Soc Nephrol. 25, 953-66. ePub

Lennon, R., Byron, A., Humphries, J.D., Randles, M.J., Carisey, A., Murphy, S., Knight, D., Brenchley, P.E., Zent, R. and Humphries, M.J. (2014). Global analysis reveals the complexity of the human glomerular extracellular matrix. J. Am. Soc. Nephrol. ePub

Mould, A.P., Craig, S.E., Byron, S.K., Humphries, M.J. and Jowitt, T.A. (2014). Disruption of integrin-fibronectin complexes by allosteric but not ligand-mimetic inhibitors. Biochem J. 464,  301-313. PubMed

Ng, D., Humphries, J.D., Byron, A., Millon-Fremillon, A., Warwood, S., Knight, D. and Humphries, M.J. Microtubule-dependent modulation of adhesion complex composition and location. eCollection. 9: e115213. PubMed

Jacquemet, G., Green, D.M., Bridgewater, R.M., von Kriegsheim, A., Humphries, M.J.,  Norman, J.C. and Caswell, P.T. (2013). RCP-driven α5β1 recycling suppresses Rac and promotes RhoA activity via the RacGAP1-IQGAP1 complex. J Cell Biol. 202, 917-35. PubMed

Byron, A., Humphries, J.D., Humphries, M.J. (2013). Defining the extracellular matrix using proteomics. Int. J. Exp. Pathol. Epub ahead of print

Jacquemet, G. and Humphries, M.J. (2013). IQGAP1 is a key node within the small GTPase network. Brief report, Small GTPases. 19, 4. PubMed

Jacquemet, G., Humphries, M.J. and Caswell, P.T. (2013). Role of adhesion receptor trafficking in 3D cell migration. 10.1016/j.ceb.2013.05.008 Curr Opin Cell Biol. PMC

Jacquemet, G., Morgan, M.R., Byron, A., Humphries, J.D., Choi, C.K., Chen, C.S., Caswell, P.T. and Humphries, M.J. (2013). Rac1 is deactivated at integrin activation sites via an IQGAP1/filamin-A/RacGAP1 pathway. J Cell Sci. in press.

Kong, F., Parks, W.M., Dumbauld, D.W., Takagi, J., Garcia, A.J., Mould, A.P., Humphries, M.J. and Zhu, C. (2013). Cyclic mechanical reinforcement of integrin-ligand interactions. Mol. Cell 49, 1060-8. sciencedirect

Soteriou, D., Iskender, B., Byron, A., Humphries, J.D., Borg-Bartolo, S., Haddock, M.C., Baxter, M.A., Knight, D., Humphries, M.J., Kimber, S.J. (2013). Comparative proteomic analysis of supportive and unsupportive extracellular matrix substrates for human embryonic stem cell maintenance. J. Biol. Chemhttp://dx.doi.org/10.1074/jbc.M113.463372

Warwood, S., Byron, A., Humphries, M.J. and Knight, D. (2013). The effect of peptide adsorption on signal linearity and a simple approach to improve reliability of quantification. J Proteomics. 85,160-4. Sciencedirect

Morgan, M.R., Hamidi, H., Bass, M.D., Warwood, S., Ballestrem C. and Humphries, M.J. (2013). Syndecan-4 phosphorylation is a control point for integrin recycling. Dev. Cell 24, 472-85. PubMed

Byron, A., Humphries, J.D., Craig, S.E., Knight, D and Humphries, M.J. (2012). Proteomic analysis of α4β1 integrin adhesion complexes reveals α-subunit-dependent protein recruitment. Proteomics 12, 2107-14. doi: 10.1002/pmic.201100487. PubMed

Byron, A., Humphries, J.D. and Humphries, M.J. (2012). Alternative cellular roles for proteins identified using proteomics. J. Proteomics 75, 4184-5. PubMed

Le Goff, M.M., Sutton, M.J., Slevin, M., Latif, A., Humphries, M.J. and Bishop, P.N. (2012). Opticin exerts its anti-angiogenic activity by regulating extracellular matrix adhesiveness. J. Biol. Chem. 287, 28027-28036. PubMed

Rashid, S.T., Humphries, J.D., Byron, A., Dhar, A., Askari, J.A., Selley, J.N., Knight, D., Goldin, R.D., Thursz, M., Humphries, M.J. (2012). Proteomic analysis of extracellular matrix from the hepatic stellate cell line LX-2 identifies CYR61 and Wnt-5a as novel constituents of fibrotic liver. J Proteome Res11, 4052-64.

Tonge, D.A., de Burgh, H.T., Docherty, R., Humphries, M.J., Craig, S.E. and Pizzey, J. (2012) Fibronectin supports neurite outgrowth and axonal regeneration of adult brain neurons in vitro. Brain Res. 1453, 8-16. PubMed

Bass, M.D., Williamson, R.C., Humphries, J.D., Byron, A., Morgan, M.R., Martin P. and Humphries, M.J. (2011). A syndecan-4 hair trigger initiates wound healing through caveolin- and RhoG-regulated integrin endocytosis. Dev. Cell 21, 681-93. PubMed

 

Byron, A., Humphries, J. D., Bass, M. D., Knight, D., & Humphries, M. J. (2011). Proteomic analysis of integrin adhesion complexes. Science Signaling, 4(167), pt2. doi:10.1126/scisignal.2001827. Sci. Signal. 4: pt2

 

Rantala, J.K., Pouwels, J., Pellinen, T., Veltel, S., Laasola, P., Potter, C.S., Duffy, T., Sundberg, J.P., Kallioniemi, O., Askari, J.A., Humphries, M., Parsons, M., Salmi, M. and Ivaska, J. (2011). SHARPIN is an endogenous inhibitor of beta1-integrin activation. Nature Cell Biol 13, 1315-24. doi: 10.1038/ncb2340. PubMed link

 

Tiwari, S., Askari, J.A., Humphries, M.J. and Bulleid, N.J. (2011). Divalent cations regulate the folding and activation status of integrins during their intracellular trafficking. J. Cell Sci. 124: 1672-1680. PubMed link

 

Worth, D. C., Hodivala-Dilke, K., Robinson, S. D., King, S. J., Morton, P. E., Gertler, F. B., Humphries, M. J., and Parsons, M. (2010) Alpha v beta3 integrin spatially regulates VASP and RIAM to control adhesion dynamics and migration, J Cell Biol 189, 369-83 PubMed link.

Askari, J. A., Tynan, C. J., Webb, S. E., Martin-Fernandez, M. L., Ballestrem, C., and Humphries, M. J. (2010) Focal adhesions are sites of integrin extension, J Cell Biol 188, 891-903 PubMed link.

Mould, A. P., Koper, E. J., Byron, A., Zahn, G., and Humphries, M. J. (2009) Mapping the ligand-binding pocket of integrin alpha5beta1 using a gain-of-function approach, Biochem J 424, 179-89 PubMed link.

Morgan, M. R., Byron, A., Humphries, M. J., and Bass, M. D. (2009) Giving off mixed signals--distinct functions of alpha5beta1 and alphavbeta3 integrins in regulating cell behaviour, IUBMB Life 61, 731-8 PubMed link.

Mahoney, C. M., Morgan, M. R., Harrison, A., Humphries, M. J., and Bass, M. D. (2009) Therapeutic ultrasound bypasses canonical syndecan-4 signaling to activate rac1, J Biol Chem 284, 8898-909 PubMed link.

Kong, F., Garcia, A. J., Mould, A. P., Humphries, M. J., and Zhu, C. (2009) Demonstration of catch bonds between an integrin and its ligand, J Cell Biol 185, 1275-84 PubMed link.

Humphries, J. D., Byron, A., Bass, M. D., Craig, S. E., Pinney, J. W., Knight, D., and Humphries, M. J. (2009) Proteomic analysis of integrin-associated complexes identifies RCC2 as a dual regulator of Rac1 and Arf6, Sci Signal 2, ra51 PubMed link.

Humphries, M. J., and Reynolds, A. (2009) Cell-to-cell contact and extracellular matrix, Curr Opin Cell Biol 21, 613-5 PubMed link.

Humphries, M. J. (2009) Cell adhesion assays, Methods Mol Biol 522, 1-8 PubMed link.

Faye, C., Moreau, C., Chautard, E., Jetne, R., Fukai, N., Ruggiero, F., Humphries, M. J., Olsen, B. R., and Ricard-Blum, S. (2009) Molecular interplay between endostatin, integrins, and heparan sulfate, J Biol Chem 284, 22029-40 PubMed link.

Deakin, N. O., Bass, M. D., Warwood, S., Schoelermann, J., Mostafavi-Pour, Z., Knight, D., Ballestrem, C., and Humphries, M. J. (2009) An integrin-α4-14-3-3ζ-paxillin ternary complex mediates localised Cdc42 activity and accelerates cell migration, J Cell Sci 122, 1654-64 PubMed link.

Byron, A., Humphries, J. D., Askari, J. A., Craig, S. E., Mould, A. P., and Humphries, M. J. (2009) Anti-integrin monoclonal antibodies, J Cell Sci 122, 4009-11 PubMed link.

Bass, M. D., Morgan, M. R., and Humphries, M. J. (2009) Syndecans shed their reputation as inert molecules, Sci Signal 2, pe18 PubMed link.

Askari, J. A., Buckley, P. A., Mould, A. P., and Humphries, M. J. (2009) Linking integrin conformation to function, J Cell Sci 122, 165-70 PubMed link.

Valdramidou, D., Humphries, M. J., and Mould, A. P. (2008) Distinct roles of beta 1 midas, admidas and limbs cation-binding sites in ligand recognition by integrin alpha 2beta 1, The Journal of biological chemistry PubMed link.

Telci, D., Wang, Z., Li, X., Verderio, E. A., Humphries, M. J., Baccarini, M., Basaga, H., and Griffin, M. (2008) Fibronectin-TG2 matrix rescues RGD-impaired cell adhesion through syndecan-4 and beta 1 integrin co-signaling, The Journal of biological chemistry PubMed link.

Parsons, M., Messent, A. J., Humphries, J. D., Deakin, N. O., and Humphries, M. J. (2008) Quantification of integrin receptor agonism by fluorescence lifetime imaging, Journal of cell science 121, 265-71 PubMed link.

Kloepper, J. E., Hendrix, S., Bodo, E., Tiede, S., Humphries, M. J., Philpott, M. P., Fassler, R., and Paus, R. (2008) Functional role of beta 1 integrin-mediated signalling in the human hair follicle, Experimental cell research 314, 498-508 PubMed link.

Humphries, S. M., Lu, Y., Canty, E. G., and Kadler, K. E. (2008) Active negative control of collagen fibrillogenesis in vivo. Intracellular cleavage of the type I procollagen propeptides in tendon fibroblasts without intracellular fibrils, J Biol Chem 283, 12129-35 PubMed link.

Bass, M. D., Morgan, M. R., Roach, K. A., Settleman, J., Goryachev, A. B., and Humphries, M. J. (2008) p190RhoGAP is the convergence point of adhesion signals from alpha 5 beta 1 integrin and syndecan-4, J Cell Biol 181, 1013-26 PubMed link.

Watson, P. M., Humphries, M. J., Relton, J., Rothwell, N. J., Verkhratsky, A., and Gibson, R. M. (2007) Integrin-binding RGD peptides induce rapid intracellular calcium increases and MAPK signaling in cortical neurons, Molecular and cellular neurosciences 34, 147-54 PubMed link.

Morgan, M. R., Humphries, M. J., and Bass, M. D. (2007) Synergistic control of cell adhesion by integrins and syndecans, Nature reviews 8, 957-69 PubMed link.

Kloepper, J. E., Hendrix, S., Bodo, E., Tiede, S., Humphries, M. J., Philpott, M. P., Fassler, R., and Paus, R. (2007) Functional role of beta1 integrin-mediated signalling in the human hair follicle, Exp Cell Res PubMed link.

Humphries, J. D., Wang, P., Streuli, C., Geiger, B., Humphries, M. J., and Ballestrem, C. (2007) Vinculin controls focal adhesion formation by direct interactions with talin and actin, The Journal of cell biology 179, 1043-57 PubMed link.

Humphries, J. D., and Humphries, M. J. (2007) CD14 is a ligand for the integrin alpha4beta1, FEBS letters 581, 757-63 PubMed link.

Bax, D. V., Mahalingam, Y., Cain, S., Mellody, K., Freeman, L., Younger, K., Shuttleworth, C. A., Humphries, M. J., Couchman, J. R., and Kielty, C. M. (2007) Cell adhesion to fibrillin-1: identification of an Arg-Gly-Asp-dependent synergy region and a heparin-binding site that regulates focal adhesion formation, Journal of cell science 120, 1383-92 PubMed link.

Bass, M. D., Roach, K. A., Morgan, M. R., Mostafavi-Pour, Z., Schoen, T., Muramatsu, T., Mayer, U., Ballestrem, C., Spatz, J. P., and Humphries, M. J. (2007) Syndecan-4-dependent Rac1 regulation determines directional migration in response to the extracellular matrix, The Journal of cell biology 177, 527-38 PubMed link.

Askari, J. A., Thornton, D. J., Humphries, J. D., Buckley, P. A., and Humphries, M. J. (2007) The alternatively spliced type III connecting segment of fibronectin is a zinc-binding module, Matrix Biol 26, 485-93 PubMed link.

Wijelath, E. S., Rahman, S., Namekata, M., Murray, J., Nishimura, T., Mostafavi-Pour, Z., Patel, Y., Suda, Y., Humphries, M. J., and Sobel, M. (2006) Heparin-II domain of fibronectin is a vascular endothelial growth factor-binding domain: enhancement of VEGF biological activity by a singular growth factor/matrix protein synergism, Circulation research 99, 853-60 PubMed link.

Turner, N. J., Murphy, M. O., Kielty, C. M., Shuttleworth, C. A., Black, R. A., Humphries, M. J., Walker, M. G., and Canfield, A. E. (2006) Alpha2(VIII) collagen substrata enhance endothelial cell retention under acute shear stress flow via an alpha2beta1 integrin-dependent mechanism: an in vitro and in vivo study, Circulation 114, 820-9 PubMed link.

Sumathipala, R., Xu, C., Seago, J., Mould, A. P., Humphries, M. J., Craig, S. E., Patel, Y., Wijelath, E. S., Sobel, M., and Rahman, S. (2006) The "linker" region (amino acids 38-47) of the disintegrin elegantin is a novel inhibitory domain of integrin alpha5beta1-dependent cell adhesion on fibronectin: evidence for the negative regulation of fibronectin synergy site biological activity, The Journal of biological chemistry 281, 37686-96 PubMed link.

Mould, A. P., McLeish, J. A., Huxley-Jones, J., Goonesinghe, A. C., Hurlstone, A. F., Boot-Handford, R. P., and Humphries, M. J. (2006) Identification of multiple integrin beta1 homologs in zebrafish (Danio rerio), BMC cell biology 7, 24 PubMed link.

Humphries, M. J., Limesand, K. H., Schneider, J. C., Nakayama, K. I., Anderson, S. M., and Reyland, M. E. (2006) Suppression of apoptosis in the protein kinase Cdelta null mouse in vivo, J Biol Chem 281, 9728-37 PubMed link.

Humphries, J. D., Byron, A., and Humphries, M. J. (2006) Integrin ligands at a glance, Journal of cell science 119, 3901-3 PubMed link.

Bauer, R., Humphries, M., Fassler, R., Winklmeier, A., Craig, S. E., and Bosserhoff, A. K. (2006) Regulation of integrin activity by MIA, The Journal of biological chemistry 281, 11669-77 PubMed link.

Wei, Y., Czekay, R. P., Robillard, L., Kugler, M. C., Zhang, F., Kim, K. K., Xiong, J. P., Humphries, M. J., and Chapman, H. A. (2005) Regulation of alpha5beta1 integrin conformation and function by urokinase receptor binding, J Cell Biol 168, 501-11 PubMed link.

Mould, A. P., Travis, M. A., Barton, S. J., Hamilton, J. A., Askari, J. A., Craig, S. E., Macdonald, P. R., Kammerer, R. A., Buckley, P. A., and Humphries, M. J. (2005) Evidence that monoclonal antibodies directed against the integrin beta subunit plexin/semaphorin/integrin domain stimulate function by inducing receptor extension, J Biol Chem 280, 4238-46 PubMed link.

Meng, X., Cheng, K., Krohkin, O., Mould, A. P., Humphries, M. J., Ens, W., Standing, K., and Wilkins, J. A. (2005) Evidence for the presence of a low-mass beta1 integrin on the cell surface, J Cell Sci 118, 4009-16 PubMed link.

Humphries, M. J., Mostafavi-Pour, Z., Morgan, M. R., Deakin, N. O., Messent, A. J., and Bass, M. D. (2005) Integrin-syndecan cooperation governs the assembly of signalling complexes during cell spreading, Novartis Found Symp 269, 178-88; discussion 188-92, 223-30 PubMed link.

Humphries, J. D., Schofield, N. R., Mostafavi-Pour, Z., Green, L. J., Garratt, A. N., Mould, A. P., and Humphries, M. J. (2005) Dual functionality of the anti-beta1 integrin antibody, 12G10, exemplifies agonistic signalling from the ligand binding pocket of integrin adhesion receptors, J Biol Chem 280, 10234-43 PubMed link.

Glasner, J., Blum, H., Wehner, V., Stilz, H. U., Humphries, J. D., Curley, G. P., Mould, A. P., Humphries, M. J., Hallmann, R., Rollinghoff, M., and Gessner, A. (2005) A small molecule alpha 4 beta 1 antagonist prevents development of murine Lyme arthritis without affecting protective immunity, J Immunol 175, 4724-34 PubMed link.

Gibson, R. M., Craig, S. E., Heenan, L., Tournier, C., and Humphries, M. J. (2005) Activation of integrin alpha5beta1 delays apoptosis of Ntera2 neuronal cells, Mol Cell Neurosci 28, 588-98 PubMed link.

Ewan, R., Huxley-Jones, J., Mould, A. P., Humphries, M. J., Robertson, D. L., and Boot-Handford, R. P. (2005) The integrins of the urochordate Ciona intestinalis provide novel insights into the molecular evolution of the vertebrate integrin family, BMC evolutionary biology 5, 31 PubMed link.

Dallas, S. L., Sivakumar, P., Jones, C. J., Chen, Q., Peters, D. M., Mosher, D. F., Humphries, M. J., and Kielty, C. M. (2005) Fibronectin regulates latent transforming growth factor-beta (TGF beta) by controlling matrix assembly of latent TGF beta-binding protein-1, J Biol Chem 280, 18871-80 PubMed link.

Clark, K., Pankov, R., Travis, M. A., Askari, J. A., Mould, A. P., Craig, S. E., Newham, P., Yamada, K. M., and Humphries, M. J. (2005) A specific alpha5beta1-integrin conformation promotes directional integrin translocation and fibronectin matrix formation, J Cell Sci 118, 291-300 PubMed link.

Bella, J., and Humphries, M. J. (2005) Calpha-H...O = C hydrogen bonds contribute to the specificity of RGD cell-adhesion interactions, BMC structural biology 5, 4 PubMed link.

Zhang, X., Craig, S. E., Kirby, H., Humphries, M. J., and Moy, V. T. (2004) Molecular basis for the dynamic strength of the integrin alpha4beta1/VCAM-1 interaction, Biophys J 87, 3470-8 PubMed link.

Travis, M. A., van der Flier, A., Kammerer, R. A., Mould, A. P., Sonnenberg, A., and Humphries, M. J. (2004) Interaction of filamin A with the integrin beta 7 cytoplasmic domain: role of alternative splicing and phosphorylation, FEBS Lett 569, 185-90 PubMed link.

Mould, A. P., and Humphries, M. J. (2004) Regulation of integrin function through conformational complexity: not simply a knee-jerk reaction?, Curr Opin Cell Biol 16, 544-51 PubMed link.

Mould, A. P., and Humphries, M. J. (2004) Cell biology: adhesion articulated, Nature 432, 27-8 PubMed link.

Humphries, M. J., Travis, M. A., Clark, K., and Mould, A. P. (2004) Mechanisms of integration of cells and extracellular matrices by integrins, Biochem Soc Trans 32, 822-5 PubMed link.

Humphries, M. J. (2004) Monoclonal antibodies as probes of integrin priming and activation, Biochem Soc Trans 32, 407-11 PubMed link.

Bax, D. V., Messent, A. J., Tart, J., van Hoang, M., Kott, J., Maciewicz, R. A., and Humphries, M. J. (2004) Integrin alpha5beta1 and ADAM-17 interact in vitro and co-localize in migrating HeLa cells, J Biol Chem 279, 22377-86 PubMed link.

Barton, S. J., Travis, M. A., Askari, J. A., Buckley, P. A., Craig, S. E., Humphries, M. J., and Mould, A. P. (2004) Novel activating and inactivating mutations in the integrin beta1 subunit A domain, Biochem J 380, 401-7 PubMed link.

Mould, A. P., Barton, S. J., Askari, J. A., Craig, S. E., and Humphries, M. J. (2003) Role of ADMIDAS cation-binding site in ligand recognition by integrin alpha 5 beta 1, J Biol Chem 278, 51622-9 PubMed link.

Mould, A. P., Symonds, E. J., Buckley, P. A., Grossmann, J. G., McEwan, P. A., Barton, S. J., Askari, J. A., Craig, S. E., Bella, J., and Humphries, M. J. (2003) Structure of an integrin-ligand complex deduced from solution x-ray scattering and site-directed mutagenesis, The Journal of biological chemistry 278, 39993-9 PubMed link.

Mould, A. P., Barton, S. J., Askari, J. A., McEwan, P. A., Buckley, P. A., Craig, S. E., and Humphries, M. J. (2003) Conformational changes in the integrin beta A domain provide a mechanism for signal transduction via hybrid domain movement, J Biol Chem 278, 17028-35 PubMed link.

Mostafavi-Pour, Z., Askari, J. A., Parkinson, S. J., Parker, P. J., Ng, T. T., and Humphries, M. J. (2003) Integrin-specific signaling pathways controlling focal adhesion formation and cell migration, J Cell Biol 161, 155-67 PubMed link.

Humphries, M. J., Symonds, E. J., and Mould, A. P. (2003) Mapping functional residues onto integrin crystal structures, Curr Opin Struct Biol 13, 236-43 PubMed link.

Humphries, M. J., McEwan, P. A., Barton, S. J., Buckley, P. A., Bella, J., and Mould, A. P. (2003) Integrin structure: heady advances in ligand binding, but activation still makes the knees wobble, Trends Biochem Sci 28, 313-20 PubMed link.

Bax, D. V., Bernard, S. E., Lomas, A., Morgan, A., Humphries, J., Shuttleworth, C. A., Humphries, M. J., and Kielty, C. M. (2003) Cell adhesion to fibrillin-1 molecules and microfibrils is mediated by alpha 5 beta 1 and alpha v beta 3 integrins, J Biol Chem 278, 34605-16 PubMed link.

Whittard, J. D., Craig, S. E., Mould, A. P., Koch, A., Pertz, O., Engel, J., and Humphries, M. J. (2002) E-cadherin is a ligand for integrin alpha2beta1, Matrix Biol 21, 525-32 PubMed link.

Santas, A. J., Peterson, J. A., Halbleib, J. L., Craig, S. E., Humphries, M. J., and Peters, D. M. (2002) Alternative splicing of the IIICS domain in fibronectin governs the role of the heparin II domain in fibrillogenesis and cell spreading, J Biol Chem 277, 13650-8 PubMed link.

Parsons, M., Keppler, M. D., Kline, A., Messent, A., Humphries, M. J., Gilchrist, R., Hart, I. R., Quittau-Prevostel, C., Hughes, W. E., Parker, P. J., and Ng, T. (2002) Site-directed perturbation of protein kinase C- integrin interaction blocks carcinoma cell chemotaxis, Mol Cell Biol 22, 5897-911 PubMed link.

Mould, A. P., Askari, J. A., Barton, S., Kline, A. D., McEwan, P. A., Craig, S. E., and Humphries, M. J. (2002) Integrin activation involves a conformational change in the alpha 1 helix of the beta subunit A-domain, J Biol Chem 277, 19800-5 PubMed link.

Humphries, M. J. (2002) Insights into integrin-ligand binding and activation from the first crystal structure, Arthritis Res 4 Suppl 3, S69-78 PubMed link.

Bass, M. D., and Humphries, M. J. (2002) Cytoplasmic interactions of syndecan-4 orchestrate adhesion receptor and growth factor receptor signalling, Biochem J 368, 1-15 PubMed link.

Aquilina, A., Korda, M., Bergelson, J. M., Humphries, M. J., Farndale, R. W., and Tuckwell, D. (2002) A novel gain-of-function mutation of the integrin alpha2 VWFA domain, European journal of biochemistry / FEBS 269, 1136-44 PubMed link.

Stallmach, A., Giese, T., Pfister, K., Wittig, B. M., Kunne, S., Humphries, M., Zeitz, M., and Meuer, S. C. (2001) Activation of beta(1) integrins mediates proliferation and inhibits apoptosis of intestinal CD4-positive lymphocytes, Eur J Immunol 31, 1228-38 PubMed link.

Mostafavi-Pour, Z., Askari, J. A., Whittard, J. D., and Humphries, M. J. (2001) Identification of a novel heparin-binding site in the alternatively spliced IIICS region of fibronectin: roles of integrins and proteoglycans in cell adhesion to fibronectin splice variants, Matrix Biol 20, 63-73 PubMed link.

Humphries, M. J., and Mould, A. P. (2001) Structure. An anthropomorphic integrin, Science 294, 316-7 PubMed link.

Humphries, M. J. (2001) Cell adhesion assays, Mol Biotechnol 18, 57-61 PubMed link.

Hicks, M. S., O'Leary, V., Wilkin, M., Bee, S. E., Humphries, M. J., and Baron, M. (2001) DrhoGEF3 encodes a new Drosophila DH domain protein that exhibits a highly dynamic embryonic expression pattern, Development genes and evolution 211, 263-7 PubMed link.

Coe, A. P., Askari, J. A., Kline, A. D., Robinson, M. K., Kirby, H., Stephens, P. E., and Humphries, M. J. (2001) Generation of a minimal alpha5beta1 integrin-Fc fragment, J Biol Chem 276, 35854-66 PubMed link.

Tuckwell, D. S., Smith, L., Korda, M., Askari, J. A., Santoso, S., Barnes, M. J., Farndale, R. W., and Humphries, M. J. (2000) Monoclonal antibodies identify residues 199-216 of the integrin alpha2 vWFA domain as a functionally important region within alpha2beta1, Biochem J 350 Pt 2, 485-93 PubMed link.

Mould, A. P., Askari, J. A., and Humphries, M. J. (2000) Molecular basis of ligand recognition by integrin alpha 5beta 1. I. Specificity of ligand binding is determined by amino acid sequences in the second and third NH2-terminal repeats of the alpha subunit, J Biol Chem 275, 20324-36 PubMed link.

Lyon, M., Rushton, G., Askari, J. A., Humphries, M. J., and Gallagher, J. T. (2000) Elucidation of the structural features of heparan sulfate important for interaction with the Hep-2 domain of fibronectin, J Biol Chem 275, 4599-606 PubMed link.

Jackson, T., Blakemore, W., Newman, J. W., Knowles, N. J., Mould, A. P., Humphries, M. J., and King, A. M. (2000) Foot-and-mouth disease virus is a ligand for the high-affinity binding conformation of integrin alpha5beta1: influence of the leucine residue within the RGDL motif on selectivity of integrin binding, J Gen Virol 81, 1383-91 PubMed link.

Humphries, J. D., Askari, J. A., Zhang, X. P., Takada, Y., Humphries, M. J., and Mould, A. P. (2000) Molecular basis of ligand recognition by integrin alpha5beta 1. II. Specificity of arg-gly-Asp binding is determined by Trp157 OF THE alpha subunit, J Biol Chem 275, 20337-45 PubMed link.

Humphries, M. J. (2000) Integrin structure, Biochem Soc Trans 28, 311-39 PubMed link.

Humphries, M. J. (2000) Integrin cell adhesion receptors and the concept of agonism, Trends Pharmacol Sci 21, 29-32 PubMed link.

Clark, K., Newham, P., Burrows, L., Askari, J. A., and Humphries, M. J. (2000) Production of recombinant soluble human integrin alpha4beta1, FEBS Lett 471, 182-6 PubMed link.

Valles, S., Tsoi, C., Huang, W. Y., Wyllie, D., Carlotti, F., Askari, J. A., Humphries, M. J., Dower, S. K., and Qwarnstrom, E. E. (1999) Recruitment of a heparan sulfate subunit to the interleukin-1 receptor complex. Regulation by fibronectin attachment, J Biol Chem 274, 20103-9 PubMed link.

Sharma, A., Askari, J. A., Humphries, M. J., Jones, E. Y., and Stuart, D. I. (1999) Crystal structure of a heparin- and integrin-binding segment of human fibronectin, EMBO J 18, 1468-79 PubMed link.

Schofield, K. P., and Humphries, M. J. (1999) Identification of fibronectin IIICS variants in human bone marrow stroma, Blood 93, 410-1 PubMed link.

Ng, T., Shima, D., Squire, A., Bastiaens, P. I., Gschmeissner, S., Humphries, M. J., and Parker, P. J. (1999) PKCalpha regulates beta1 integrin-dependent cell motility through association and control of integrin traffic, EMBO J 18, 3909-23 PubMed link.

Ng, T. T., Collins, I. E., Kanner, S. B., Humphries, M. J., Amft, N., Wickremasinghe, R. G., D'Cruz, D., Nye, K. E., and Morrow, W. J. (1999) Integrin signalling defects in T-lymphocytes in systemic lupus erythematosus, Lupus 8, 39-51 PubMed link.

McCormick, C. J., Tuckwell, D. S., Crisanti, A., Humphries, M. J., and Hollingdale, M. R. (1999) Identification of heparin as a ligand for the A-domain of Plasmodium falciparum thrombospondin-related adhesion protein, Mol Biochem Parasitol 100, 111-24 PubMed link.

Mastrangelo, A. M., Homan, S. M., Humphries, M. J., and LaFlamme, S. E. (1999) Amino acid motifs required for isolated beta cytoplasmic domains to regulate 'in trans' beta1 integrin conformation and function in cell attachment, J Cell Sci 112 ( Pt 2), 217-29 PubMed link.

Humphries, M. J. (1999) Towards a structural model of an integrin, Biochem Soc Symp 65, 63-78 PubMed link.

Curley, G. P., Blum, H., and Humphries, M. J. (1999) Integrin antagonists, Cell Mol Life Sci 56, 427-41 PubMed link.

Burrows, L., Clark, K., Mould, A. P., and Humphries, M. J. (1999) Fine mapping of inhibitory anti-alpha5 monoclonal antibody epitopes that differentially affect integrin-ligand binding, Biochem J 344 Pt 2, 527-33 PubMed link.

Watson, R. E., Parry, E. J., Humphries, J. D., Jones, C. J., Polson, D. W., Kielty, C. M., and Griffiths, C. E. (1998) Fibrillin microfibrils are reduced in skin exhibiting striae distensae, Br J Dermatol 138, 931-7 PubMed link.

Schofield, K. P., Humphries, M. J., de Wynter, E., Testa, N., and Gallagher, J. T. (1998) The effect of alpha4 beta1-integrin binding sequences of fibronectin on growth of cells from human hematopoietic progenitors, Blood 91, 3230-8 PubMed link.

Retta, S. F., Balzac, F., Ferraris, P., Belkin, A. M., Fassler, R., Humphries, M. J., De Leo, G., Silengo, L., and Tarone, G. (1998) beta1-integrin cytoplasmic subdomains involved in dominant negative function, Mol Biol Cell 9, 715-31 PubMed link.

Newham, P., Craig, S. E., Clark, K., Mould, A. P., and Humphries, M. J. (1998) Analysis of ligand-induced and ligand-attenuated epitopes on the leukocyte integrin alpha4beta1: VCAM-1, mucosal addressin cell adhesion molecule-1, and fibronectin induce distinct conformational changes, J Immunol 160, 4508-17 PubMed link.

Mould, A. P., Garratt, A. N., Puzon-McLaughlin, W., Takada, Y., and Humphries, M. J. (1998) Regulation of integrin function: evidence that bivalent-cation-induced conformational changes lead to the unmasking of ligand-binding sites within integrin alpha5 beta1, Biochem J 331 ( Pt 3), 821-8 PubMed link.

Mould, A. P., Burrows, L., and Humphries, M. J. (1998) Identification of amino acid residues that form part of the ligand-binding pocket of integrin alpha5 beta1, J Biol Chem 273, 25664-72 PubMed link.

Messent, A. J., Tuckwell, D. S., Knauper, V., Humphries, M. J., Murphy, G., and Gavrilovic, J. (1998) Effects of collagenase-cleavage of type I collagen on alpha2beta1 integrin-mediated cell adhesion, J Cell Sci 111 ( Pt 8), 1127-35 PubMed link.

McCutcheon, J. C., Hart, S. P., Canning, M., Ross, K., Humphries, M. J., and Dransfield, I. (1998) Regulation of macrophage phagocytosis of apoptotic neutrophils by adhesion to fibronectin, J Leukoc Biol 64, 600-7 PubMed link.

Humphries, M. J., and Newham, P. (1998) The structure of cell-adhesion molecules, Trends Cell Biol 8, 78-83 PubMed link.

Green, L. J., Mould, A. P., and Humphries, M. J. (1998) The integrin beta subunit, Int J Biochem Cell Biol 30, 179-84 PubMed link.

Globus, R. K., Doty, S. B., Lull, J. C., Holmuhamedov, E., Humphries, M. J., and Damsky, C. H. (1998) Fibronectin is a survival factor for differentiated osteoblasts, J Cell Sci 111 ( Pt 10), 1385-93 PubMed link.

Fernandez, C., Clark, K., Burrows, L., Schofield, N. R., and Humphries, M. J. (1998) Regulation of the extracellular ligand binding activity of integrins, Front Biosci 3, d684-700 PubMed link.

Tuckwell, D. S., Xu, Y., Newham, P., Humphries, M. J., and Volanakis, J. E. (1997) Surface loops adjacent to the cation-binding site of the complement factor B von Willebrand factor type A module determine C3b binding specificity, Biochemistry 36, 6605-13 PubMed link.

Tuckwell, D. S., and Humphries, M. J. (1997) A structure prediction for the ligand-binding region of the integrin beta subunit: evidence for the presence of a von Willebrand factor A domain, FEBS Lett 400, 297-303 PubMed link.

Tselepis, V. H., Green, L. J., and Humphries, M. J. (1997) An RGD to LDV motif conversion within the disintegrin kistrin generates an integrin antagonist that retains potency but exhibits altered receptor specificity. Evidence for a functional equivalence of acidic integrin-binding motifs, J Biol Chem 272, 21341-8 PubMed link.

Schofield, K. P., Rushton, G., Humphries, M. J., Dexter, T. M., and Gallagher, J. T. (1997) Influence of interleukin-3 and other growth factors on alpha4beta1 integrin-mediated adhesion and migration of human hematopoietic progenitor cells, Blood 90, 1858-66 PubMed link.

Ng, T. T., Kanner, S. B., Humphries, M. J., Wickremasinghe, R. G., Nye, K. E., Anderson, J., Khoo, S. H., and Morrow, W. J. (1997) The integrin-triggered rescue of T lymphocyte apoptosis is blocked in HIV-1-infected individuals, J Immunol 158, 2984-99 PubMed link.

Newham, P., Craig, S. E., Seddon, G. N., Schofield, N. R., Rees, A., Edwards, R. M., Jones, E. Y., and Humphries, M. J. (1997) Alpha4 integrin binding interfaces on VCAM-1 and MAdCAM-1. Integrin binding footprints identify accessory binding sites that play a role in integrin specificity, J Biol Chem 272, 19429-40 PubMed link.

Mould, A. P., Askari, J. A., Aota, S., Yamada, K. M., Irie, A., Takada, Y., Mardon, H. J., and Humphries, M. J. (1997) Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 and anti-beta1 monoclonal antibodies. Evidence that the synergy sequence of fibronectin is recognized by the amino-terminal repeats of the alpha5 subunit, J Biol Chem 272, 17283-92 PubMed link.

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